Sanitary importance of arcobacter

• Autores: Arturo Alberto Levican Asenjo
• Directores de la Tesis: Luis Roberto Collado González (dir. tes.), María José Figueras Salvat (dir. tes.)
• Lectura: En la Universitat Rovira i Virgili ( España ) en 2013
• Idioma: inglés
• Tribunal Calificador de la Tesis: Jesús Angel López Romalde (presid.), Josepa Gené Díaz (secret.), Sandra Mclellan (voc.)
• Materias:
  • Ciencias de la vida
    • Microbiología
      • Bacteriología
  • Ciencias médicas
    • Ciencias clínicas
      • Microbiología clínica
• Enlaces
  • Tesis en acceso abierto en: TDX
• Resumen

  • The genus Arcobacter, belongs to the family Campylobacteraceae, along with. Some species, are considered emerging pathogens and have been associated with gastrointestinal diseases and bacteraemia in humans and with diarrhoea, mastitis and abortions in animals. Diarrhoea, is the most common clinical presentation in humans. However, still are not fully defined the routes of transmission, the virulence factors present in pathogenic strains have not been determined and the isolation and identification methods are still deficient. All of these factors contribute to a possible underestimation of the sanitary importance of this genus. In this thesis it has been demonstrated the existence of five new species of this genus. In their differentiation, new tools have been used, such as the Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Multilocus Phylogenetic Analysis (MLPA) with concatenated 5 genes (rpoB, gyrB, hsp60, gyrA and atpA). Furthermore, the performance of 5 molecular identification methods was compared and none of them was useful for all the strains studied. In this regard, the updating of 16S rRNARFLP method for the identification of all species proved very useful. Moreover, in this thesis was a great diversity of species of this genus in samples of shellfish and sewage. Both matrices have epidemiological importance; however, they have been poorly studied. It was also demonstrated that the use in parallel of direct culturing and post enrichment, as well as the incubation under aerobic and microaerophilic, enhances the recovery and diversity of arcobacters. Regarding the virulence of Arcobacter spp., most of them proved to be potential enteropathogens for man, as they showed the presence of virulence factors and were able to adhere and invade human intestinal cells (Caco-2). Finally, it was demonstrated that Arcobacter may be confused with Campylobacter sp., generating an underestimation of their sanitary importance.