Effects of grape seed procyanidin extract on proliferation and apoptosis in pancreatic cells

• Autores: Lidia Cedó
• Directores de la Tesis: Montserrat Pinent Armengol (dir. tes.)
• Lectura: En la Universitat Rovira i Virgili ( España ) en 2013
• Idioma: inglés
• Tribunal Calificador de la Tesis: Lluís Arola Ferrer (presid.), Ana María Rodríguez Guerrero (secret.), Jamileh Movassat (voc.)
• Materias:
  • Química
    • Bioquímica
      • Biología molecular
  • Ciencias tecnológicas
    • Tecnología de los alimentos
      • Propiedades de los alimentos
• Enlaces
  • Tesis en acceso abierto en: TDX
• Resumen

  • Procyanidins have been reported to modulate glucose homeostasis and ?-cell functionality. However, their effects on pancreatic cell mass remain unknown. Therefore, this doctoral thesis focused on the study of the effects of a grape seed procyanidin extract (GSPE) on proliferation and apoptosis processes in pancreatic cells. Our results indicate that although the extract did not affect these processes under healthy conditions, it modulated ?-cell proliferation and apoptosis under altered conditions. In vitro, GSPE enhanced the pro-apoptotic effects of high glucose and were antiproliferative under high glucose, insulin, and palmitate levels in the INS-1E cell line. In vivo, the effects of the extract depended on the dose, the duration of the treatment, and/or the gender. However, the extract tended to counteract the deleterious effects of the cafeteria diet or the Zucker Fatty genotype. Concerning the adenocarcinoma cell line MIA PaCa-2, GSPE exerted antiproliferative and pro-apoptotic effects, demonstrating anti-carcinogenic activity.