Dental care process characterization spectroscopic methods for a robust determination of remineralization and dental sensitivity /
- Autores: Olga Kotkowska
- Directores de la Tesis: Manuel Valiente Malmagro (dir. tes.)
- Lectura: En la Universitat Autònoma de Barcelona ( España ) en 2016
- Idioma: inglés
- Tribunal Calificador de la Tesis: Jordi Coello Bonilla (presid.), Igors Sics (secret.), Maher Al-Atari Abou-Asi (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: DDD
- Resumen
In presented dissertation a problem of hypersensitivity and remineralization/demineralization problem is treated. A novel, scientific tools for the evaluation of the efficacy of products targeting the hypersensitivity or caries creation were proposed. Moreover, experiments implementing FTIR spectroscopy as well as synchrotron techniques for the dental tissue investigation were performed. In order to design a proper sealing material and to investigate its efficacy, the porosity of the dentine has to be determined. In the present dissertation a novel methodology for the evaluation of the efficacy of the hypersensitivity treatment has been developed. It is presented in the first part of the work and is based on the dentin disc model methodology. Specimens treated with a desensitizing agent were observed under Scanning Electron Microscope (SEM) and the resulting images were analyzed by the MATLAB automatic segmentation algorithm developed to calculate the number and area of the open tubuli. The present method is a suitable scientific tool that will allow scientists and private companies the in vivo monitoring and assistance on the development of new remineralising agents. The comparison of three commercial toothpastes presented in this work showed a successful application of the developed methodology and proved it to be suitable for that kind of comparative studies. The proceeding parts of the presented dissertation were focused on the problem of the demineralization and opposite-remineralisation processes of teeth. In the second part of presented PhD thesis a novel approach implementing the Hyper Spectral Imaging (HSI) technique for the evaluation of the efficacy of the remineralising treatment is presented. Hyper Spectral Imaging (HSI) with two types of NIR cameras (161 and 256 channels) and confocal Raman microscopy (CRM) as a complementary technique were applied for analysis of teeth before and after application of different remineralizing agents. Obtained results were carefully analyzed and compared by the implementation of a MATLAB routine that used the correlation coefficient and MCR data analysis in order to obtain necessary information from the acquired spectra of dental samples. Taking into consideration the simplicity of the presented method it can be a powerful tool to obtain basic information about the performed treatment. In the process of the evaluation of a new dental product, this kind of information can lead to the decision whether to continue the development of the product or to proceed with another, more promising approach. In the third part of the presented dissertation the Infrared Spectroscopic Ellipsometry (IRSE) and synchrotron ellipsometry is proposed for the determination of the optical constants of human dental tissues. Human teeth were treated with the remineralising agent and analyzed with the synchrotron IR ellipsometer. Furtherly, a series of experiments and simulations were performed in order to obtain the most suitable reference sample for the interpretation of the obtained synchrotron data. In the last ¿ fourth part, structural changes in the human enamel and dentin induced by the remineralization process were investigated. The spectra from various points proceeding from different locations of the tooth were compared and studied by the FTIR technique. Obtained data were interpreted applying Principal Component Analysis (PCA) and curve-fitting procedures. Obtained results were compared to the reference hydroxyl and fluorapatite powders and scientific literature.
