Función del gen drybp durante el desarrollo de drosophila y su regulación mediada por micrornas

• Autores: Ricardo Aparicio Crespo
• Directores de la Tesis: Ana de Busturia y Jimeno (dir. tes.), Ernesto Sánchez-Herrero Arbide (tut. tes.)
• Lectura: En la Universidad Autónoma de Madrid ( España ) en 2012
• Idioma: español
• Tribunal Calificador de la Tesis: José Fernández Piqueras (presid.), María Inmaculada González García (secret.), David Gubb (voc.), Carmela Cales Bourdet (voc.), Miguel Ángel Vidal Caballero (voc.)
• Materias:
  • Ciencias de la vida
    • Biología molecular
• Enlaces
  • Tesis en acceso abierto en: digitool-uam.greendata.es
• Resumen

  • Summary Controlled spatial and temporal regulation of gene expression is required for animal development and survival. Once gene transcriptional states have been established, their maintenance during cellular proliferation is crucial for the organism.

    The Polycomb (PcG) and the trithorax (trxG) groups of genes play a pivotal role in this process. The PcG and trxG genes were first identified in Drosophila melanogaster, due to their role in morphogenesis as regulators of homeotic gene expression. However, studies in vertebrates, as well as emerging studies in Drosophila support the idea that the PcG and trxG genes also have significant roles in other biological processes, such as hematopoiesis, stem cell renewal, control of cell proliferation and tumorigenesis.

    In this Thesis, it has been studied the function of dRYBP (drosophila Ring and YY1 binding protein), a PcG and trxG associated protein, in the development of Drosophila.

    The conclusions of this Thesis work are the following: 1) The dRYBP proteín regulates gene expression through its interaction with the Polycomb Response Elements of the target genes. 2) The polyhomeotic (ph) and the pleiohomeotic (pho) genes, both of the PcG, participate in the hematopoiesis of Drosophila regulating the proliferation and/or the diferentation of the ¿Crystal cells¿. Low levels of PHO produce a decrease in the number of Crystal cells¿, while both low and high levels of PH produce an increase in the number of ¿Crystal cells¿ . 3) The dRYBP gene participates in the hematopoiesis of Drosophila regulating the total number of hemocites controlling their apoptosis/proliferation. 4) The dRYBP gene, which is expressed in the immune-competent tissues, regulates the immune response of Drosophila. Under immune challenge, the dRYBP gene negatively regulates the immune response controlled by the Imd pathway. 5) The dRYBP protein does not transcriptionally control the expression of the Imd pathway components. 6) The dRYBP protein functions together with SKPA to inhibit the immune response. The repression can take place at different levels of the Imd pathway: at the Imd level and at the Relish level. 7) High levels of expression of miR-7, miR-284 y miR-306 induce a decrease in the wing size. 8) The levels of expression of dRYBP are regulated by miR-7, which is expressed in the boundary cells of the wing imaginal disc. 9) High levels of dRYBP repress the Notch signalling pathway in the non boundary cells of the wing imaginal disc, and 10) miR-7 controls Notch signalling pathway at the dorso-ventral boundary to regulate the size of the wing imaginal disc.