Regulation of small non-coding RNAs during oocyte differentiation in teleost gonads: providing the embryo with the capacity to produce proteins

• Autores: Joyanta Bir
• Directores de la Tesis: Ibon Cancio Uriarte (dir. tes.), Maren Ortiz Zarragoitia (codir. tes.)
• Lectura: En la Universidad del País Vasco - Euskal Herriko Unibertsitatea ( España ) en 2025
• Idioma: inglés
• Programa de doctorado: Programa de Doctorado en Medio Ambiente y Recursos Marinos por la Universidad del País Vasco/Euskal Herriko Unibertsitatea
• Calificación: Sobresaliente
• Materias:
  • Ciencias de la vida
• Enlaces
  • Tesis en acceso abierto en: ADDI
• Resumen

  • Marine environments are increasingly threatened by climate change and chemical contamination, including complex mixtures of pollutants such as endocrine-discrupting compounds (EDCs). EDCs can induce feminization and intersex condition in male fish, characterized by the presence of oocytes within testicular tissue, ultimately threatening the reproductive success at both individual and population levels. Understanding how EDCs induce oocyte development in testis is crucial to decipher the molecular mechanisms behind the intersex condition. In fish, oocyte quality is defined by the ability to regulate protein and lipid synthesis, essential for early embryonic development. Ribosome biogenesis, which involves the synthesis of rRNAs, eibosomal proteins, tRNAs, and small non-coding RNAs, is among the most energy-demanding cellular processes and determines the oocyte's capacity to grow, mature, and support the embryo. During teleost oogenesis, small non-coding RNAs play a key role in preparing the oocyte for early embryonic development by accumulating ribosomal intermediates, tRNAs, and ribonucleoprotein particles in the ooplasm. These components are fully activated only after fertilization, ensuring that the embryo can synthesize proteins before zygotic genone activation. In the context, this PhD project investigates the molecular process controlling the expression of core protein synthesis machinery, including small non-coding RNAs and RNAP-III regulatory factors to provide a comprehensive framework for understanding teleost oocyte differentiation and growth...Molecular profiling of these RNAs provides a precise and quantitative alternative marker to traditional histology for assessing ovarian differentiation and responses to environmental stressors such as xenoestrogens, with broad applications in reproductive biology, aquaculture, and fisheries management.