Study of dna-ion interactions using nanopore translocation and optical tweezers

• Autores: Alejandro Colchero Truniger
• Directores de la Tesis: Fèlix Ritort Farran (dir. tes.), Isabel Pastor del Compo (codir. tes.), Giancarlo Franzese (tut. tes.)
• Lectura: En la Universitat de Barcelona ( España ) en 2025
• Idioma: español
• Tribunal Calificador de la Tesis: Ulrich Felix Keyser (presid.), Antonio Diego Alcaraz González (secret.), Aleksandra Radanović (voc.)
• Programa de doctorado: Programa de Doctorado en Física por la Universidad de Barcelona
• Materias:
  • Física
    • Óptica
      • Láseres
  • Ciencias de la vida
    • Biofísica
    • Biología molecular
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• Resumen

  • This thesis employs two complementary single-molecule techniques, nanopipette microscopy, and optical tweezers, to investigate the impact of high concentrations of various monovalent salts on DNA. The thesis starts by characterizing nanopipettes' conductance and noise properties over a broad range of concentrations. Once nanopipettes have been characterized, they are used to conduct DNA translocation experiments to examine how different cations influence DNA translocation parameters. These experiments also allow us to explore the effects of applied voltage and salt concentration on the folding configurations of DNA during translocation.

    Moreover, we explore why compact DNA folding configurations exhibit lower dwell times compared to more extended folding configurations. Finally, optical tweezers are used to perform unzipping experiments on a DNA hairpin, providing insights into the stability of DNA under high ionic strength conditions relating to the DNA translocation experiments.