Design, synthesis, and evaluation of optical chemosensors to detect potential biomarkers of pathological conditions
- Autores: Mariana Barros Nogueira
- Directores de la Tesis: Pablo Gaviña Costero (dir. tes.), Ana Maria Costero Nieto (codir. tes.)
- Lectura: En la Universitat de València ( España ) en 2024
- Idioma: inglés
- Número de páginas: 276
- Tribunal Calificador de la Tesis: Margarita Parra Álvarez (presid.), Juan Felipe Miravet Celades (secret.), Carlos Lodeiro Espiño (voc.)
- Programa de doctorado: Programa de Doctorado en Química por la Universitat de València (Estudi General) y la Universitat Politècnica de València
- Materias:
- Enlaces
- Tesis en acceso abierto en: RODERIC
- Resumen
This PhD thesis entitled "Design, Synthesis, and Evaluation of Optical Chemosensors to Detect Potential Biomarkers of Pathological Conditions" consists of two parts that make innovative advancements in biomarker detection and controlled-release nanomaterials.
Part 1 focuses on synthesizing and characterizing two new sensors designed to distinguish between Spermine (Spm) and Spermidine (Spd). Chapter 3 introduces a new TPE derivative with a molecular cleft disposition that exhibits significant fluorescence enhancement in response to Spm and Spd. Theoretical results emphasize the coordination strength associated with positive charges, highlighting the specificity of the sensor for flexible molecules with a large number of amino groups. Chapter 4 discusses the development of a hybrid organic-inorganic material (S1) using MCM-41 particles. The material has a molecular gate based on N-hydroxysuccinimide that can detect Spm and Spd in solution and within RAW 264.7 macrophages. These findings demonstrate the potential of this system for sensitive and selective biomarker detection and suggest possible advancements in cancer diagnosis for urine samples and cellular contexts.
Part 2 focuses on the development of controlled-release nanomaterials. Chapter 8 discusses the design of molecular gates responsive to hypoxia for targeted drug delivery using mesoporous silica nanoparticles. Material S2 is particularly noteworthy as it is responsive to hypoxic conditions, making it an ideal platform for controlled drug release. Chapter 9 introduces a novel nitro derivative-based molecular gate for making liposomes that are attuned to hypoxia-responsive applications. Chapter 10 explores the molecular recognition and protein labeling of three push-pull heterocyclic dyes, laying the groundwork for potential applications, including interactions with the HIF protein. This section emphasizes the interdisciplinary nature of advanced drug delivery and biomedical applications, seamlessly integrating molecular gates, selective dyes for proteins, and liposome systems.
