Efecto antimicrobiano de tres soluciones irrigantes sobre enterococcus faecalis alojados en el conducto radicular

• Autores: Gabriela Martin
• Directores de la Tesis: María Rosa del Carmen Visvisián (dir. tes.), María Gabriela Paraje (codir. tes.)
• Lectura: En la Universidad Nacional de Córdoba (UNC) ( Argentina ) en 2014
• Idioma: español
• Enlaces
  • Tesis en acceso abierto en: hdl.handle.net
• Resumen
  • español

    Con el propósito de evaluar in vitro el efecto antibacteriano de diferentes soluciones irrigantes sobre E.faecalis en el conducto radicular, se realizaron 2 estudios.- 1, evaluación del efecto inmediato y a distancia de distintos irrigantes de uso endodóntico, en dientes de edades conocidas infectados con E.faescalis. 2- evaluación de la permeabilidad dentaria E.faecalis con dos grupos etarios. Para tal fín se seleccionaron 106 piezas dentarias humanas extraídas, las cuales se dividieron en dos grupos etarios: A: menores de 25 años y B: mayores de 50 años

  • English

    The aim of the present study was to evaluate in vitro the effectiveness of different irrigating solutions on E.faecalis in root canals. Two studies were developed:

    1.- evaluation of immediate and long term residual effects of different endodontic irrigants on E.faecalis biofilms in root canals. 2.- evaluation of dentin permeability to E.faecalis in two age groups. 106 freshly extracted human teeth were selected for this experiment, distributed into 2 groups, according to the patient age: A- younger than 25 year old and B- older than 50 year old. After adequate preparation of the teeth, 88 premolars were used for the first study, that were infected with E.faecalis ATCC 29212 and incubated for 60 days at 37º. Following this incubation, the premolars were instrumented with ProTaper system and divided into 6 groups according to irrigation protocols: 1-NaOCl 1% + EDTA; 2-NaOCl 2,5% + EDTA; 3-NaOCl 5,25% + EDTA;

    4-NaOCl 1% + MTAD; 5-NaOCl 1% + EDTA + IPI; 6-NaOCl 1% + EDTA + NaOCl 5,25 %. Five bacteriological samples were taken from the root canals: the first one before instrumentation and irrigation, the second one immediately after the final irrigation; the third, fourth and fifth one at 2, 7 and 30 days. The samples were plated on agar and incubated at 37º for 48 hours. The colony forming units (CFUs) grown were counted. For the second study, 18 canines were selected, that were divided into 2 age groups. A cavity was done on the external root surface to eliminate radicular cementum and they were mounted into an experimental model of two sealed flasks.

    The root canals were infected with E.faecalis through the access cavity in the upper flask and the lower one was filled with sterile growth broth. The turbidity of the lower flask was checked for 30 days and it indicated positive culture. The statistical analyses was done by ANOVA test, Kruskal- Wallis and Mann-Whitney test for the first study;

    and Wilcoxon test was applied for the second study. In the first study, premolars younger than 25 year old revealed higher values of CFU/ml of E.faecalis than older specimens. The antibacterial immediate study demonstrated that NaOCl 1% + EDTA + IPI was the most effective protocol to reduce E.faecalis in root canals, followed by:

    NaOCl 1% + EDTA + NaOCl 5,25%; NaOCl 1% + MTAD; NaOCl 5,25% + EDTA;

    NaOCl 2,5% + EDTA and NaOCl 1% + EDTA. Root canal reinfection was not observed with NaOCl 1% + EDTA + IPI; NaOCl 1% + EDTA and NaOCl 2,5% + EDTA at 30 days. In the second study, all canines younger than 25 year old revealed dentin permeability to E.faecalis, while only 78% of 50 year old specimens. It could be concluded that although E.faecalis was not totally eradicated with any irrigation protocol, the tested irrigating solutions were effective to reduce E.faecalis in the root canals. The final rinse with an antibacterial solution increased root canal disinfection.