CDK-mediated phosphorylation of Yku80 and its role in DNA repair
- Autores: María de los Reyes Carballar Ruiz
- Directores de la Tesis: Josep Clotet (dir. tes.), Javier Jiménez Jiménez (dir. tes.)
- Lectura: En la Universitat Internacional de Catalunya ( España ) en 2021
- Idioma: español
- Tribunal Calificador de la Tesis: Martí Aldea Malo (presid.), Pablo Huertas Sánchez (secret.), Miguel González Blanco (voc.)
- Programa de doctorado: Programa de Doctorado en Ciencias de la Salud por la Universitat Internacional de Catalunya
- Calificación: Sobresaliente Cum Laude
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
DNA double-strand breaks (DSBs) are considered the most deleterious lesions of DNA and they are repaired by means of two main mechanisms: homologous recombination (HR) and non-homologous end-joining (NHEJ). These mechanisms are regulated throughout the cell cycle being HR restricted to the S/G2 phases while NHEJ is active during the different phases of the cell cycle. Here I presented evidence of NHEJ regulation by CDK phosphorylation of one of the key proteins of the NHEJ repair pathway, Yku80. Yku80 is phosphorylated both in vitro and in vivo by the CDK Pho85 in association with the G1 cyclin Pcl1. A non phosphorylatable version of Yku80 (yku80-S623A) shows increased NHEJ activity, reduced HR events and higher sensitivity upon bleomycin treatment, a DSB-inducing agent, specifically when DNA damage was induced during the G2 phase of the cell cycle. Interestingly, the overexpression of the non-phosphorylatable version of human Ku80 (Ku80T629A) increased bleomycin sensitivity in different cancer lines suggesting Ku80 phosphorylation and its role in DSB repair regulation might be conserved. Thus, the results presented in this work provide evidence of a new mechanism to regulate DSB repair pathway choice by CDK-mediated phosphorylation of Yku80.
