Development of smart devices for the detection of metabolites of toxic substances and disease-related enzyme overexpression
- Autores: Marcia Domínguez Rodríguez
- Directores de la Tesis: Ramón Martínez Máñez (dir. tes.), Juan Francisco Blandez Barradas (dir. tes.)
- Lectura: En la Universitat Politècnica de València ( España ) en 2023
- Idioma: inglés
- Tribunal Calificador de la Tesis: Ana Belén Descalzo López (presid.), Miguel Angel Gonzalez Martinez (secret.), Rafael Ballesteros Garrido (voc.)
- Programa de doctorado: Programa de Doctorado en Química por la Universitat de València (Estudi General) y la Universitat Politècnica de València
- Materias:
- Enlaces
- Tesis en acceso abierto en: RiuNet
- Resumen
This PhD thesis focuses on the development of smart devices for detecting metabolites of toxic substances and enzyme overexpression related to diseases. It addresses the limitations of conventional diagnostic techniques and highlights the usefulness of urine as a biological sample. Concepts of mesoporous silica materials, molecular recognition, and fluorogenic molecular probes are discussed. The objectives are outlined, and then a nanodevice for detecting trans, trans-muconic acid (t,t-MA) in urine (S4) is presented. A near-infrared fluorogenic probe for the detection of high levels of alanine aminopeptidase in urine (NB-ALA) is described. New probes for detecting cancer biomarkers (NB-SO3-Leu and NB-SO3-Ala) are also introduced, emphasizing the importance of their solubility and renal elimination. Finally, a non-invasive molecular probe (Cy7-MAO) is proposed for detecting monoamine oxidase enzyme through fluorescence measurements in urine. The conclusions underscore the potential of these systems for the diagnosis and treatment of diseases.
