Development of gene-activated matrices as tools in new regenerative therapies
- Autores: Héctor Rilo Alvarez
- Directores de la Tesis: Marcos García Fuentes (dir. tes.), Anxo Vidal Figueroa (dir. tes.)
- Lectura: En la Universidade de Santiago de Compostela ( España ) en 2024
- Idioma: inglés
- Tribunal Calificador de la Tesis: Rosa María Hernández Martín (presid.), Luis Antonio Díaz Gómez (secret.), María del Carmen Arufe Gonda (voc.)
- Programa de doctorado: Programa de Doctorado en Investigación y Desarrollo de Medicamentos por la Universidad de Santiago de Compostela
- Materias:
- Enlaces
- Tesis en acceso abierto en: MINERVA
- Resumen
The main goal of this thesis has been the development, optimization, and testing of three-dimensional mRNA-activated matrices as a system for the administration of transcription factors for articular cartilage regeneration. Modifications in the mRNA synthesis provided higher stability and efficacy as compared to standard mRNA. TAMs chondrogenic effects were tested in 3D cultures activated with SOX9, TGF-β3 or combinations of both. The transcript combination provided the best effects as it combined the strong chondrogenic effect of TGF-β3 with the capacity of SOX9 to promote chondroblast differentiation while inhibiting fibrotic tissue formation. The two best TAM prototypes were studied in a cartilage defect rabbit model where showed no signs of toxicity and promoted the expression of specific tissue markers.
