Complejos intracelulares formados por la RNA polimerasa del virus de la gripe: oligomerización, factores asociados y complementación génica

• Autores: Nuria Jorba Grifols
• Directores de la Tesis: Juan Ortín (dir. tes.)
• Lectura: En la Universidad Autónoma de Madrid ( España ) en 2009
• Idioma: español
• Tribunal Calificador de la Tesis: Esteban Domingo Solans (presid.), José María Almendral del Río (secret.), Susana de la Luna Gargantilla (voc.), José Francisco Parra Fernández (voc.), Juan Antonio García Alvarez (voc.)
• Calificación: Sobresaliente Cum Laude
• Materias:
  • Ciencias de la vida
    • Biología molecular
• Enlaces
  • Tesis en acceso abierto en: Biblos-e Archivo
• Resumen

  • The influenza virus polymerase complex is a heterotrimer formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. In this Doctoral Thesis we present the characterization of the complexes formed intracellularly by the influenza polymerase in human cells. The three subunits of the polymerase were co-expressed in human cells and the intracellular complexes were purified by the TAP approach, which involves IgG-Sepharose and calmodulin-agarose chromatography, under very mild conditions.