Regulation of carbohydrate metabolism upon osmostress
- Autores: Anna Pijuan Calvet
- Directores de la Tesis: Eulàlia de Nadal Clanchet (codir. tes.), Francesc Posas Garriga (codir. tes.)
- Lectura: En la Universitat Pompeu Fabra ( España ) en 2021
- Idioma: español
- Tribunal Calificador de la Tesis: Javier Jiménez Jiménez (presid.), Antonio Casamayor Gracia (secret.), Gemma Bellí Martínez (voc.)
- Programa de doctorado: Programa de Doctorado en Biomedicina por la Universidad Pompeu Fabra
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
Yeast deal with continuous environmental perturbations that compromise its cell fitness. Upon osmostress, HOG pathway becomes activated and leads the adaptation response. To compensate the osmotic imbalance, Hog1 is in charge of the accumulation of intracellular glycerol by closing its export and promoting the synthesis. This thesis aimed to provide a deeper understanding of how Hog1 regulates carbohydrate metabolism upon osmostress. To this end, we performed an in vitro genetic-biochemical screening to identify novel Hog1 targets. We detected Pfk27, a phosphofructokinase in charge of synthetizing Fru-2,6-P2, as one of the most interesting candidates. We characterized how Pfk27 is phosphorylated and activated by Rck2 in a Hog1-dependent manner. Furthermore, we described the importance of Pfk27 in the proper metabolic response to osmostress by activating glycolysis. Finally, we study how Pfk27 mutations affect cellular fitness both in basal and stress conditions. In summary, this thesis provides a new mechanism describing a novel HOG pathway regulation of carbohydrate metabolism upon osmostress.
