A mechano-perfusion bioreactor for tissue engineering
- Autores: Dino Freitas
- Directores de la Tesis: Joquim de Ciurana Gay (dir. tes.), Paulo Jorge da Silva Bártolo (codir. tes.)
- Lectura: En la Universitat de Girona ( España ) en 2019
- Idioma: español
- Tribunal Calificador de la Tesis: Teresa Puig Miquel (presid.), Antonio Jesus Guerra Guerra (secret.), Henrique Almeida (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
Tissue Engineering plays a vital role in tissue construct to repair, maintain or replace tissues. Those tissues can be cultivated in vivo or in vitro using devices such as bioreactors. There are several approaches to create the necessary tissues, but one of the most popular and successful is by using scaffold constructs to provide the required stability and support. After the cells being implanted on the scaffolds, they are then inserted in the bioreactors.
Those bioreactors seek to mimic the conditions provided to cells by the human body. This issue by itself presents several challenges where it is required, to bioreactors, besides the optimum environment in terms of temperature, nutrients, the creation of the necessary stimulus to cells to differentiate and proliferate.
In this work, is presented a novel concept of bioreactor for Tissue Engineering that can provide multiples stimulus when cultivating the tissue. To achieve an optimised design was performed several numerical simulations to access the best design parameters. For this, it was taken into account several variables such as fluid velocity, the proximity of the inlet/outlet to the scaffold, directions of the fluid and the impact of the liquid on the scaffold and subsequently the cells by analysing the wall shear stress provoked by the fluid flow.
