Principles of design and operation in genetic and biochemical systems. Adaptive responses of yea
- Autores: Ester Vilariño Terre
- Directores de la Tesis: A. Sorribas Tello (dir. tes.)
- Lectura: En la Universitat de Lleida ( España ) en 2007
- Idioma: español
- Tribunal Calificador de la Tesis: Enrique Herrero Perpiñan (presid.), Néstor Torres Darias (secret.), José Enrique Pérez Ortín (voc.), Juli Peretó (voc.), Léon Brenig (voc.)
- Materias:
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- Resumen
The aim of this thesis is the development of methodological tools focused on the identification of principies of design and operation in genètic and biochemical systems. First, a mathematical model of the central metabolism of yeast allows the in silico exploration of the performance of hypothetical gene expression changes after a heat shock. Under the hypothesis that exist several evolutive pressures that shaped the actual adaptive profile, the alternative scenarios thatfulfill all the constraints are compared with those that do not. This allows answering the question that will be the leitmotiv of this thesis: Why a given design or operational response has been selected instead of others? The criteria that conform the definition of performance were used to evaluate each of the hypothetical's gene expression profiles and also those providing from microarray experiments with several enyironmental stresses.
Second, among all the experimental conditions, only heat shock conditions were selected. Although this specificity, stress responses trigger some changes that are common. For instance in most of them, the celi protein content changes in order to tune the effectiveness of the performance. The most econòmic is a response, the higher chance of being selected. And this ¡s reflected ¡n the over-all protein properties such as the molecular weight.
Third, theoretical approximations with mathematical models permit test
