Parámetros de regeneración del disco de ala de drosophila tras inducción de muerte celular (parameters of drosophila wing disc regeneration after cell death induction)

• Autores: Cora Bergantiños Crespo
• Directores de la Tesis: Florenci Serras Rigalt (dir. tes.)
• Lectura: En la Universitat de Barcelona ( España ) en 2012
• Idioma: español
• Tribunal Calificador de la Tesis: Jaume Baguñà Monjo (presid.), Antonio Baonza Cuenca (secret.), Marco Milán Kalbfleisch (voc.)
• Materias:
  • Ciencias de la vida
    • Genética
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• Resumen

  • The main objective of this work is to describe the basic parameters of Drosophila wing disc regeneration inducing the injury by a non-invasive method. This allows the study of regeneration in situ, avoiding the distortions produced by the cut and implantation technique. To this aim we have proposed two main goals: 1. Design and describe an alternative method to study regeneration in Drosophila wing imaginal discs inducing the injury by genetic ablation and without the need for mechanical manipulation or microsurgery. We have named this method as Cell Death Induced Regeneration (CDIR). To this aim we have accomplished the following objectives: 1.1. Test whether the Gal4/Gal80ts system is able to drive specific expression of a proapoptotic gene allowing an effective spatial and temporal control of cell death induction.

    1.2. Establish the conditions in which broad cell death induction and regeneration of the lost tissue can take place.

    1.3. Describe both, the cell death induction and the early regenerative response that follows in terms of healing and proliferation.

    1.4. Test the possible role of the Jun Kinase N-terminal (JNK) pathway driving the early regenerative response.

    2. Describe the basic parameters of cellular behaviour during regeneration elicited by the CDIR method. To achieve that purpose we have accomplished the following objectives: 2.1. Analyze the process of re-patternig that occurs during regeneration induced by cell death, using different vein and intervein markers and lacZ lineages.

    2.2. Define the basic cell behaviour after CDIR in terms of growth and orientation using the clonal analysis technique.

    2.3. Test the possible role of morphogenes and JNK pathway driving regenerative growth and re-patterning during regeneration after cell death induction.