Synthesis of n-glycan mimetics and their evaluation as c-type lectin receptor (clr) antagonists using microarray technology

• Autores: Anna Cioce
• Directores de la Tesis: Niels-Christian Reichardt (dir. tes.), Claudio Palomo Nicolau (dir. tes.), Jesús Jiménez Barbero (dir. tes.)
• Lectura: En la Universidad del País Vasco - Euskal Herriko Unibertsitatea ( España ) en 2019
• Idioma: inglés
• Tribunal Calificador de la Tesis: Mikel Oiarbide (presid.), Francisco Javier Rojo Marcos (secret.), Sabine L. Flitsch (voc.)
• Programa de doctorado: Programa de Doctorado en Química Sintética e Industrial por la Universidad del País Vasco/Euskal Herriko Unibertsitatea y la Universidad Pública de Navarra
• Materias:
  • Química
    • Bioquímica
      • Inmunoquímica
    • Química macromolecular
      • Polisacáridos
    • Química orgánica
      • Química de los hidratos de carbono
• Enlaces
  • Tesis en acceso abierto en: ADDI
• Resumen

  • Carbohydrate microarrays are well established tools for high-throughput analysis of carbohydrate-protein interactions and for screening the specificity of carbohydrate processing enzymes, In addition, a few groups have also explored the on-chip synthesis of ligands. The use of small amounts of substrates and the absence of long purification step make the microarray a time-saving approach for the parallel synthesis of hundred scaffolds.We developed a versatile platform to quickly synthesize significant number of glycomimetics via chemoenzymatic protocols and test them as potential ligands for C-type lectin receptors (CLRs). The transparent and the conductive hydrophobic-ITO surface allows to perform binding studies with labeled lectins by fluorescent spectroscopy as well as to estimate conversion rates of the on-chip reactions for every printed ligand by MALDI-TOF MS.