Transition metal-promoted bioorthogonal transformations in biological media

• Autores: Paolo Destito
• Directores de la Tesis: José Luis Mascareñas Cid (dir. tes.), Fernando José López García (codir. tes.)
• Lectura: En la Universidade de Santiago de Compostela ( España ) en 2019
• Idioma: inglés
• Tribunal Calificador de la Tesis: Eduardo Fernandez Megia (presid.), Aitziber López Cortajarena (secret.), Jérôme Waser (voc.)
• Programa de doctorado: Programa de Doctorado en Ciencia y Tecnología Química por la Universidad de Santiago de Compostela y la Universidad de Vigo
• Materias:
  • Química
    • Química orgánica
      • Química de los hidratos de carbono
      • Compuestos heterocíclicos
      • Compuestos organometálicos
      • Estructura de moléculas orgánicas
• Texto completo no disponible (Saber más ...)
• Resumen

  • This PhD thesis describes the reactivity of Au, Pd and Ru organometallic complexes in biological media. In particualar, the reactivity of Au(I) complexes was studied for the hydroarylation reaction of fluorogenic coumarins in aqueous mixture, together with its bioorthogonality and translation to living cells. Moreover, homogenous and heterogenous Pd(0) complexes were used for the allyloxycarbonyl, propargyloxycarbonyl, propargyl groups removal in aqueous media in the presence or inside living cells. The reactivity of heterogenous palladium systems was also tested towards much more challening Suzuki-Miyaura reaction in aqueous media and under biologically relevant conditions. Finally, Ru(II) complexes were used for the annulation between azide and thioalkyne in aqueous mixture, demonstrating the robustness of the reaction in the presence of biologically relevant molecules and in the presence of living bacteria. Moreover, new phoactivable Ru(II) complexes were also active for this transformation and it was possible to use them for the modification of biomolecules like DNA and small peptides.