Photobacterium damselae subsp. damselae: analysis of the genetic diversity and characterization of a conserved two-component system regulating virulence

• Autores: Mateus de Souza Terceti
• Directores de la Tesis: Carlos Rodríguez Osorio (dir. tes.)
• Lectura: En la Universidade de Santiago de Compostela ( España ) en 2018
• Idioma: inglés
• Tribunal Calificador de la Tesis: Jesús Manuel Aparicio Fernández (presid.), Trinidad de Miguel Bouzas (secret.), Sandra Manuela Rodrigues Sousa (voc.)
• Programa de doctorado: Programa de Doctorado en Avances en Biología Microbiana y Parasitaria por la Universidad de Santiago de Compostela
• Materias:
  • Ciencias de la vida
    • Microbiología
      • Bacteriología
• Enlaces
  • Tesis en acceso abierto en: MINERVA
• Resumen

  • Photobacterium damselae subsp. damselae is an emerging pathogen of marine aquaculture. By combining phenotypic and genotypic data, and comparative genomics analyses, we here show that this pathogen exhibits a high genetic diversity and that outbreaks in fish are caused by multiclonal populations. We identified and characterized the RstAB two-component signal transduction system, a major positive regulator of virulence and of many aspects of cell physiology in this bacterium. The RstAB regulon comprises the major cytotoxins Dly, PhlyP and PhlyC, as well as hitherto unreported proteins secreted by the type two secretion system (T2SS), that may constitute novel virulence factors in this pathogen.