C. Elegans integrator complex: identification and analysis of a role beyond snrna processing

• Autores: Beatriz Sáenz Narciso
• Directores de la Tesis: Juan Cabello Pardos (dir. tes.), Eva Gómez Orte (codir. tes.), María de la Paz Sacristán Martín (tut. tes.)
• Lectura: En la Universidad de Salamanca ( España ) en 2017
• Idioma: español
• Tribunal Calificador de la Tesis: Anton Gartner (presid.), Dionisio Miguel Martín Zanca (secret.), Julian Ceron Madrigal (voc.)
• Programa de doctorado: Programa de Doctorado en Biociencias: Biología y Clínica del Cáncer y Medicina Traslacional por la Universidad de Salamanca
• Materias:
  • Ciencias de la vida
    • Genética
    • Biología molecular
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• Resumen

  • In this work we described the C. elegans Integrator complex and the involvement of its INTS-6 subunit in DNA damage response.

    The Integrator complex, which is comprised of at least fourteen subunits in human cells, is responsible for snRNA 3’-end processing. In addition, some of its subunits are involved in other steps of the RNAP II transcription cycle or other biological processes such as development or DNA repair.

    Here, we demonstrated that the C. elegans Integrator complex is comprised of, at least, eleven subunits (INTS-1, INTS-2, INTS-3, INTS-4, INTS-5, INTS-6, INTS-7, INTS-8, INTS-9, INTS-11 and INTS-13). RNAi knockdown of any subunit leads to 3’- end processing defects that result in the formation of chimeric RNAs composed of an snRNA and an mRNA, which we have called “sn-mRNAs”’.

    We also detected these chimeric “sn-mRNAs” upon gamma radiation. Finally, involvement of INTS-6 in DNA repair via the HR pathway was demonstrated and we suggested a link between INTS-6 in DSB DNA repair and the formation of chimeric “sn-mRNAs”.