Producción de prebióticos galactooligosacáridos mediante técnicas biotecnológicas y de inmovilización enzimática
- Autores: Isabel María González Delgado
- Directores de la Tesis: María José López Muñoz (dir. tes.), Gabriel Morales Sánchez (codir. tes.)
- Lectura: En la Universidad Rey Juan Carlos ( España ) en 2017
- Idioma: español
- Tribunal Calificador de la Tesis: David Pedro Serrano Granados (presid.), Luis Fernando Bautista Santa Cruz (secret.), Francisco José Plou Gasca (voc.), María Elia Tomas Pejo (voc.), Birgitte Zeuner (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: TESEO
- Resumen
The present Doctoral Thesis stablishes the starting point to a new research line about Food Biotechnology with the aim of optimizing the production of prebiotic galactooligosaccharides (GOS) by enzymatic catalysis. This research is also related to the synthesis and functionalization with organic groups of porous silica materials as enzyme immobilization supports.
In order to fulfil this general aim, several partial objectives have been successfully addressed: • The establishment of optimum reaction conditions for GOS synthesis (tri-, tetra-GOS) using different β-galactosidase enzymes, applying an experimental design methodology.
• The application of recombinant DNA technology for the production of the thermostable β-galactosidase from Sulfolobus solfataricus, and the application of site-directed mutagenesis in order to improve its catalytic performance.
• Characterization of engineered sialidases from Trypanosoma rangeli for the production of sialylated human milk oligosaccharides (HMOs).
• The covalent glyoxyl immobilization on different silica supports of commercial and recombinant enzymes in order to improve the biocatalyst reusability and/or properties.
