Nuevos determinantes moleculares en la respuesta a isquemia cerebral

• Autores: María Carmen Gubern Mérida
• Directores de la Tesis: Judith Mallolas Giménez (dir. tes.), Mar Castellanos Rodrigo (codir. tes.)
• Lectura: En la Universitat de Girona ( España ) en 2015
• Idioma: español
• Tribunal Calificador de la Tesis: Teresa Gasull Dalmau (presid.), Elisabeth Kádár García (secret.), Olivia Hurtado Moreno (voc.)
• Materias:
  • Química
    • Bioquímica
      • Biología molecular
      • Proteínas
  • Ciencias médicas
    • Medicina interna
      • Neurología
• Enlaces
  • Tesis en acceso abierto en: TDX
• Resumen

  • Cerebral ischaemia is a potent inducer of gene expression, however, little is known about the mechanisms that regulate this expression. Studies of gene expression and the study of the mechanisms regulating this expression allow identifying new molecules regulated by ischaemia and possibly involved in the physiopathology of stroke. This thesis shows, (1) the importance of validating suitable endogenous controls for the studies of gene expression for each experimental condition; (2)that Gcf2/Lrrfip1 presents as a gene with diverse isoforms that are differentially expressed in response to stroke and that rLrrfip1, which has a possible protector role, is the main isoform; and (3) that the differential expression of the microRNAs remains from the acute to the chronic phase of ischaemia, having determined that miR-347 promotes neuronal apoptosis and Acsl4 and Arf3, potential targets of microRNAs differentially expressed in response to ischaemia, are new proteins to study in the physiopathology of stroke.