Información General

• Autores: Luca Nerva (ed. lit.), Walter Chitarra (ed. lit.)
• Editores: Springer-Verlag usa, Humana Press
• Año de publicación: 2026
• Colecciones: Methods in Molecular Biology, 3076
• País: Estados Unidos
• Idioma: inglés
• ISBN: 978-1-0716-5551-1
• Texto completo no disponible (Saber más ...)

Resumen

• This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology

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• Red de Bibliotecas Universitarias (REBIUN)

Listado de artículos

• • Botrytis cinerea Isolation Methods

    Crucitti Dalila, Pacifico Davide

    págs. 1-6

    

  • Culture and Maintenance Methods for Botrytis cinerea

    Crucitti Dalila, Pacifico Davide

    págs. 7-11

    

  • In Vitro Sexual Reproduction of Botrytis cinerea

    Dian Zheng, Jan A. L. van Kan

    págs. 13-19

    

  • Viable Storage of Botrytis Conidia and Sclerotia

    Frank P.J. Pieterse, Jan A.L. van Kan

    págs. 21-26

    

  • Fluorescence and Ultrastructural Microscopy for Botrytis cinerea

    Kui Liu, Xinhua Huang, Yong Chen, Boqiang Li, Shiping Tian, Tong Chen

    págs. 27-35

    

  • Cell Biology of Botrytis: Live-Cell Imaging Techniques and Protein Localization Protocols

    H.M. Suraj, Andre Fleissner, Jan A.L. van Kan

    págs. 37-43

    

  • Methods for Inoculating Botrytis cinerea on Leaves and Fruit

    Daoguo Chen, Tong Chen, Boqiang Li, Yong Chen, Shiping Tian

    págs. 45-52

    

  • Protocol for the Extraction of High-Quality DNA from Botrytis cinerea

    Chen Yan, Liu Yiqing, Jin Weibo

    págs. 53-58

    

  • Strain Differentiation by SSR-PCR Using Capillary Sequencer and Agarose Gel Electrophoresis

    Louis Backmann, Maren Scharfenberger Schmeer

    págs. 59-65

    

  • Genome Assembly and Annotation of a Plant Pathogen Using the Example of Botrytis cinerea

    Xiaoqian Shi Kunne, Jan A.L. van Kan

    págs. 67-79

    

  • Identification of Mycoviral Infections

    Ana Ruiz Padilla, Julio Luis Rodríguez Romero, Marco Chiapello, Mª A. Ayllón

    págs. 81-109

    

  • Botrytis cinerea Protoplasts Production and Inoculation

    Javier Pardo Medina, Julián Méndez García, Laura Córdoba, Mª A. Ayllón

    págs. 111-127

    

  • Assessing Sensitivity to Antifungal Plant Compounds or Agrochemicals

    H.M. Suraj, Andre Fleissner, Jan A.L. van Kan

    págs. 129-138

    

  • Extracellular Vesicle Isolation from Botrytis cinerea Culture and B. cinerea-Infected Arabidopsis Plants

    Lida Halilovic, Simoné Murguia, Yuan Wang, Huaitong Wu, Hailing Jin-

    págs. 139-147

    

  • Assays of Antifungal Activity of an Antimicrobial Peptide Against Botrytis cinerea

    Min Zhang, Po Hu, Hailing Jin-

    págs. 149-160

    

  • Evaluation of Biocontrol Efficacy by Competing Yeasts

    Antonio Domenico Marsico, Margherita D’Amico, Falvia Angela Maria Maggiolini

    págs. 161-167

    

  • Methods for Evaluating the Efficacy of Microbial Biocontrol Agents Against Botrytis cinerea on Tomato Plant

    Jean-François Bourgeay, Marc Bardin

    págs. 169-179

    

  • Procedures for the Study of Botrytis cinerea Proteome

    Almudena Escobar Niño, Olivier Coste, Maria Victoria Calcis Marzán, Francisco Javier Fernández Acero

    págs. 181-194