Signifcance of concurrent evaluation of HER2 gene amplifcation and p53 and Ki67 expression in gastric cancer tissues

• Su nan Wang ^[2] ; Yang kun Wang ^[3] ; Chao ya Zhu ^[1] ; Bo Jiang ^[4] ; Dong feng Ge ^[5] ; Ying ying Li ^[2]
  1. [1] Zhengzhou University

     Zhengzhou University

     China

     
  2. [2] Shenzhen Polytechnic, Xilihu Town, Nanshan District, Shenzhen 518055, Guangdong, China
  3. [3] Department of Pathology, The Fourth People’s Hospital of Longgang District, Shenzhen 518123, China
  4. [4] Department of Pathology, No. 990 Hospital of the PLA Joint Logistics Support Force, Zhumadian 463000, China
  5. [5] Department of Pathology, The First Afliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Jianxi District, Luoyang 471000, China

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• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 27, Nº. 1, 2025, págs. 126-134
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Objective The primary objective of this study is to explore the signifcance of concurrent evaluation of HER2 gene amplifcation and p53 and Ki67 expression in gastric cancer tissues.

    Methods Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) methodologies were used to detect HER2 gene amplifcation, as well as the expression levels of HER2, p53, and Ki67 proteins, across a group of 78 gastric cancer cases.

    Results The expression rate of the HER2 protein was determined to be 43.6% (34/78), with 17.9% (14/78) categorized as HER2 protein 3+, 14.1% (11/78) as HER2 protein 2+, and 11.5% (9/78) as HER2 protein 1+. Using FISH technology, the HER2 gene amplifcation rate was identifed as 19.2% (15/78), including 3 cases of HER2 gene cluster amplifcation, 5 cases of large granular amplifcation, 4 cases of punctate amplifcation, and 3 cases of high polysomy. The positive rate of p53 in gastric cancer cells was 52.6% (41/78), with 62.8% (49/78) of patients exhibiting a ki67 proliferation index≤30, and 37.2% (29/78) accounting for a ki67 proliferation index>30. The expression rates of the HER2 gene, p53, and ki67 in gastric cancer tissues were signifcantly associated with both gastric cancer staging and lymph node metastasis (P<0.05).

    Conclusion The HER2 gene amplifcation rate and gene copy number exhibit a positive correlation with the expression rates of p53 and ki67. Combining these assessments can provide crucial insights into the assessment of metastatic potential, disease progression, and prognosis of gastric tumor cells. This holds paramount importance in steering the formulation of individualized treatment strategies.