Establishment of callus culture of Melientha suavis Pierre

• Autores: Duangporn Premjet, Abraham K. Obeng, Siripong Premjet
• Localización: Agricultura técnica, ISSN-e 0718-5839, ISSN 0365-2807, Vol. 80, Nº. 3, 2020, págs. 459-465
• Idioma: inglés
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• Resumen

  • ABSTRACT Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis.