Genome analysis of vB_SupP_AX, a novel N4-like phage infecting Sulfitobacter

• Yundan Liu ^[1] ; Chengrui Zhu ^[1] ; Yantao Liang ^[1] ; Andrew McMinn ^[1] ; Kaiyang Zheng ^[1] ; Ziyue Wang ^[1] ; Hongmin Wang ^[1] ; Linyi Ren ^[1] ; Hongbing Shao ^[1] ; Yeong Yik Sung ^[2] ; Wen Jye Mok ^[2] ; Li Lian Wong ^[2] ; Min Wang ^[1]
  1. [1] Ocean University of China

     Ocean University of China

     China

     
  2. [2] Universiti Malaysia Terengganu

     Universiti Malaysia Terengganu

     Malasia

     
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 27, Nº. 4, 2024, págs. 1297-1306
• Idioma: inglés
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• Resumen

  • Sulfitobacter is a bacterium recognized for its production of AMP-independent sulfite oxidase, which is instrumental in the creation of sulfite biosensors. This capability underscores its ecological and economic relevance. In this study, we present a newly discovered phage, Sulfitobacter phage vB_SupP_AX, which was isolated from Maidao of Qingdao, China. The vB_SupP_AX genome is linear and double-stranded and measures 75,445 bp with a GC content of 49%. It encompasses four transfer RNA (tRNA) sequences and 79 open reading frames (ORFs), one of which is an auxiliary metabolic gene encoding thioredoxin. Consistent with other N4-like phages, vB_SupP_AX possesses three distinct RNA polymerases and is characterized by the presence of four tRNA molecules. Comparative genomic and phylogenetic analyses position vB_SupP_AX and three other viral genomes from the Integrated Microbial Genomes/Virus v4 database within the Rhodovirinae virus subfamily. The identification of vB_SupP_AX enhances our understanding of virus-host interactions within marine ecosystems.