Chloroquine regulates the lipopolysaccharide-induced inflammatory response in RAW264.7 cells

Natsuki Ota; Shoya Endo; Kouki Honma; Kuninori Iwayama; Hiroshi Yamashita; Ryosuke Tatsunami; Keisuke Sako

Ayuda

Chloroquine regulates the lipopolysaccharide-induced inflammatory response in RAW264.7 cells

Natsuki Ota ^[1] ; Shoya Endo ^[1] ; Kouki Honma ^[1] ; Kuninori Iwayama ^[1] ; Hiroshi Yamashita ^[1] ; Ryosuke Tatsunami ^[1] ; Keisuke Sato ^[1]
1. [1] Hokkaido University of Science
  
  Hokkaido University of Science
  
  Chūō-ku, Japón
Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 52, Nº. 4, 2024, págs. 97-103
Idioma: inglés
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Resumen
- Introduction and objectives: Macrophage-induced inflammation plays a key role in defense against injury and harmful pathogens. Autophagy and the inflammatory response are associated; however, the relationship between the autophagy pathway and lipopolysaccharide (LPS)- induced inflammatory responses remains unknown. We aimed to determine the effect of autophagy on the LPS-induced myeloid differentiation factor 88 (MyD88)/nuclear transcription factor kB (NF-kB) pathway-mediated inflammatory response in RAW264.7 cells.
  
  Materials and Methods: To determine the effect of autophagy on the LPS-induced inflammatory response, using various in vitro assays, we determined the effect of autophagy inhibitors and inducers on the inflammatory response in RAW264.7 cells.
  
  Results: Chloroquine (CQ), an autophagy inhibitor, suppressed pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor α (TNFα) in LPS-stimulated RAW264.7 cells. CQ also affected inflammatory mediators such as myeloid differentiation factor 88 and NF-kB in LPS-stimulated RAW264.7 cells.
  
  Conclusion: This study demonstrated that CQ regulates the LPS-induced inflammatory response in RAW264.7 cells. We propose that targeting the regulation of pro-inflammatory cytokine levels and inflammatory mediators using CQ is a promising therapeutic approach for preventing inflammatory injury. CQ serves as a potential therapeutic target for treating various inflammatory diseases.