The genome of Epiperipatus biolleyi (Peripatidae), a Costa rican onychophoran

• Mora, Marielos ^[1] ; Herrera, Alvaro ^[2] ; León, Pedro ^[3]
  1. [1] Universidad de Costa Rica

     Universidad de Costa Rica

     Hospital, Costa Rica

     
  2. [2] Centro de Investigación en Biología Celular y Molecular
  3. [3] Centro de Investigación en Biología Celular y Molecular. Escuela de Medicina,Universidad de Costa Rica

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• Localización: Revista de Biología Tropical, ISSN 0034-7744, Vol. 44, Nº. 1, 1996 (Ejemplar dedicado a: Volume 44 – Regular number 1 – April 1996; 19–22)
• Idioma: inglés
• Títulos paralelos:
  • The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
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  • español

    Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome.

  • English

    Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome.