Seroprevalence of T. cruzi infection in Canis familiaris, state of Sucre, Venezuela

• Berrizbeitia, Mariolga ^[1] ; Concepcion, Juan Luis ; Carzola, Valentina ^[1] ; Rodríguez, Jessicca ^[1] ; Cáceres, Ana ^[2] ; Quiñones, Wilfredo ^[2]
  1. [1] Universidad de Oriente

     Universidad de Oriente

     Venezuela

     
  2. [2] Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de los Andes, Mérida, Venezuela
• Localización: Biomédica. Revista del Instituto Nacional de Salud, ISSN-e 2590-7379, ISSN 0120-4157, Vol. 33, Nº. 2, 2013, págs. 214-225
• Idioma: español
• Títulos paralelos:
  • Seroprevalence of T. cruzi infection in Canis familiaris, Sucre state, Venezuela
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• Resumen
  • español

    Introduction: Trypanosoma cruzi infection in humans has been extensively studied in Venezuela; however, in reservoirs it has been less investigated.Objective: The objective of this study was to determine the seroepidemiology of T. cruzi in the state of Sucre, Venezuela.Materials and methods: A cross-sectional and prospective study conducted in 95 towns and 577 dwellings in the 15 municipalies of the state of Sucre, Venezuela, from August to November, 2008. The evaluation of serum samples was performed with the CruziELISA kit and the multiple antigens binding assays (MABA). Furthermore, epidemiological surveys were applied to evaluate the risk factors.Results: A total of dogs (average age of 2, 6 + 2.2 years, 226 males and 137 females) was evaluated. The combination of the ELISA / MABA tests detected 78 positive sera, sixty-nine negative and 10 of inconclusive results. The seroprevalence of the T. cruzi infection in dogs in the state of Sucre, was 22.1% (CI 95%: 20.58-22.4%). No significant statistic association was found between the T. cruzi infection in dogs and the evaluated epidemiological variables: hunting dogs that slept oudoors roaming freely in the populated center, sex of the animal and eating habits. The T. cruzi infection was associated to the age of canines, being significantly higher in the group of 0 to 3 years, when compared with older dogs.Conclusions: The high T. cruzi seroprevalence dected in dogs shows that in this región of Venezuela there prevails an important risk factor of transmissibility of this parasite to human populations.doi: http://dx.doi.org/10.7705/biomedica.v33i2.760 

  • English

    Introduction. T. cruzi infections in humans have been extensively studied in Venezuela, however, the reservoirs of the disease have been little studied. Objective. The aim of this study was to determine the seroepidemiology of T. cruzi in dogs in Sucre state, Venezuela. Materials and methods. This was a cross-sectional and prospective study conducted in 95 towns and 577 dwellings in 15 municipalities of Sucre state, Venezuela, from August to November 2008. Serum samples from dogs were tested by the CruziELISA kit and the multiple antigens binding assay (MABA). In addition, epidemiological surveys were undertaken to evaluate the risk factors. Results. We surveyed dogs from 576 houses in 95 rural towns in the15 municipalities of Sucre state. A total of 363 dogs were evaluated (mean age: 2.6 ± 2.2 years, male: 226, female: 137). The combination of the ELISA / MABA detected 78 positive sera, sixty nine negative and 10 inconclusive results. This gave a seroprevalence for T. cruzi infection in dogs in Sucre state of 22.1% (95% CI: 20.58-22.4%). There was no statistically significant association between dogs infected with T. cruzi and the following epidemiological variables: hunting dogs, dogs which sleep outdoors and wander freely around the town, sex of the dog or their eating habits. T. cruzi infection was associated with age, being significantly higher in dogs aged 0 to 3 years as compared to the older groups. Conclusions. The high T. cruzi seroprevalence detected in dogs shows that this represents a high risk factor for the transmission of this parasite to human populations. doi: http://dx.doi.org/10.7705/biomedica.v33i2.760