Resumen de Evaluación de la germinación y vigor de alfalfa (Medicago sativa L.) Con rizobacterias y CuSO4·5H₂O
Mario Francisco Santoyo de la Cruz, Abdul Khalil Gardezi, Guillermo Carrillo Castañeda, Hector Manuel Ortega Escobar, Oscar Raúl Mancilla Villa, Juan Enrique Rubiños Panta, Jose Abel Lopez Buenfil, Mario U. Larqué Saavedra, Gabriel Haro Aguilar, Cristian Alejandro Ali Gamboa
Metallic contaminants in soil and water represent a serious growing problem due to anthropogenic activities. Some phytoremediation techniques with rhizobacteria can be used as an alternative solution to the decontamination of sites affected by metals. The objective of this work was to evaluate the germination of alfalfa seed in solutions with seven concentrations of copper sulphate and to analyse the vigour and germination of Alfalfa inoculated with rhizobacteria. Alfalfa seeds were germinated in copper sulphate solutions, the seeds were inoculated with bacterial suspensions of two strains of Pseudomonas M40 and M67 and the germination percentages were determined, and vigour tests were made. The highest percentage of germination was achieved after seven days in the solutions of copper sulphate 10-4 to 10-5 M, the inoculation of Alfalfa seeds with the strain M40 presented 91.3% germination in two days and the variety Aragón inoculated with the strain M40 it presented greater stem growth. The higher the concentration of copper sulphate, the less germination occurs, however, if it manages to tolerate small concentrations of copper sulphate. Medicago sativa is a viable alternative for the decontamination of sites affected by potentially toxic metals.
