Evaluation of EDARADD (rs79233817) Gene Polymorphism in Children with Dental Caries Compared to Caries-Free Controls

MohammadHassan Lotfalizadeh; Forough Taheri; Sepehr Kobravi; Aida Jafari; Zahra Pirmoradi; Kasra Nazari; Mehri Safari; Atefe Ali Moradi; Akram Goharifar; Farzaneh Lal Alizadeh

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Evaluation of EDARADD (rs79233817) Gene Polymorphism in Children with Dental Caries Compared to Caries-Free Controls

MohammadHassan Lotfalizadeh ^[1] ; Forough Taheri ^[2] ; Sepehr Kobravi ^[7] ; Aida Jafari ^[8] ; Zahra Pirmoradi ^[3] ; Kasra Nazari ^[3] ; Mehri Safari ^[9] ; Atefe Ali Moradi ^[4] ; Akram Goharifar ^[5] ; Farzaneh lal Alizadeh ^[6]
1. [1] North Khorasan University of Medical Sciences
  
  North Khorasan University of Medical Sciences
  
  Irán
2. [2] Tehran University of Medical Sciences
  
  Tehran University of Medical Sciences
  
  Irán
3. [3] Zhengzhou University
  
  Zhengzhou University
  
  China
4. [4] Tabriz University of Medical Sciences
  
  Tabriz University of Medical Sciences
  
  Irán
5. [5] Islamic Azad University
  
  Islamic Azad University
  
  Irán
6. [6] Mashhad University of Medical Sciences
  
  Mashhad University of Medical Sciences
  
  Irán
7. [7] Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tehran Azad University, Tehran, Iran.
8. [8] Faculty of Dentistry (Khorasgan), Isfahan Azad University, Isfahan, Iran.
9. [9] Operative Dentistry Specialist, Department of Operative Dentistry, Dental School, Islamic Azad University of Isfahan branch, Isfahan, Iran.
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Localización: Academic Journal of Health Sciences: Medicina Balear, ISSN-e 2255-0560, Vol. 39, Nº. 3, 2024, págs. 40-44
Idioma: inglés
Títulos paralelos:
- Evaluación del polimorfismo del gen EDARADD (rs79233817) en niños con caries dental comparados con controles libres de caries
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Resumen
- español
  Objetivo: La caries dental es una enfermedad compleja que resulta de una combinación de factores genéticos y ambientales.
  
  Se ha descubierto que el gen EDARADD desempeña un papel en el desarrollo de los dientes y se ha asociado a diversos rasgos dentales. En este estudio, nuestro objetivo fue examinar la posible correlación entre el polimorfismo del gen EDARADD (rs79233817) y la susceptibilidad a la caries dental.
  
  Métodos: El estudio incluyó un total de 400 participantes, de los cuales 200 eran individuos con caries dental y 200 controles libres de caries. El genotipado del polimorfismo del gen EDARADD se realizó mediante el método PCR del sistema de mutación refractaria por amplificación de tetraprimer.
  
  Resultados: Se observó que los individuos con caries dental presentaban una frecuencia significativamente mayor del alelo A (alelo menor) para rs79233817 en comparación con los controles. Se determinó que la odds ratio (intervalo de confianza del 95%) era de 0,745 (0,624-0,751), con un valor p de 0,002. El SNP específico (rs79233817) se asoció con un riesgo elevado de caries dental (DC) tanto en el modelo genético codominante como en el dominante. Las odds ratio se calcularon en 0,45 (0,27-0,75) y 0,41 (0,26-0,72) para los respectivos modelos, con valores p correspondientes de 0,012 y 0,02.
  
  Conclusiones: Se puede inferir que el polimorfismo del gen EDARADD (rs79233817) desempeña potencialmente un papel en la susceptibilidad genética a la caries dental. Para validar y profundizar en estos hallazgos, es necesario realizar estudios adicionales con muestras de mayor tamaño en poblaciones diversas. Esto ayudará a establecer la solidez de los resultados y a investigar más a fondo los mecanismos subyacentes implicados.
- English
  Objective: Dental caries is a complex condition that results from a combination of genetic and environmental factors. The EDARADD gene has been found to play a role in the teeth development and has been associated with various dental traits. In this study, our objective was to examine the potential correlation between the EDARADD gene polymorphism (rs79233817) and susceptibility to dental carie.
  
  Methods: The study included a total of 400 participants, comprising 200 individuals with dental caries and 200 caries-free controls. The genotyping of the EDARADD gene polymorphism was performed using the tetra-primer amplification refractory mutation system PCR metho.
  
  Results: Individuals with dental caries were found to have a meaningfully higher frequency of the A allele (minor allele) for rs79233817 compared to controls. The odds ratio (95% confidence interval) was determined to be 0.745 (0.624-0.751), with a p-value of 0.002. The specific SNP (rs79233817) was associated with an elevated risk of dental caries (DC) in both the co-dominant and dominant genetic models. The odds ratios were calculated as 0.45 (0.27-0.75) and 0.41 (0.26-0.72) for the respective models, with corresponding p-values of 0.012 and 0.02.
  
  Conclusions: It can be inferred that the EDARADD gene polymorphism (rs79233817) potentially plays a role in the genetic susceptibility to dental caries. To validate and delve deeper into these findings, it is necessary to conduct additional studies with larger sample sizes in diverse populations. This will help to establish the robustness of the results and further investigate the underlying mechanisms involved.