Resumen de Estudio histopatologico en ratones infectados experimentalmente con trypanosoma cruzi

Claudio Zuniga, M. Adriana Parra, Hamilton Vela, Maria Teresita Courcelles, Ramon Vargas, Ulises Vergara

• español

  Se infectaron cuatro cepas de ratones, machos y hembras, con 2000 trypomastigotes sanguíneos de la cepa Tulahuen de Trypanosoma cruzi. Los animales de las cepas A.Sw y HTI se comportaron como resistentes y los de las cepas A/Sn y HTG como susceptibles, independiente del sexo de los animales infectados. En los casos de las cepas A.Sw, HTG y HTI se observó niveles significativamente más altos de parasitemia en los machos. A nivel histopatológico el daño tisular no presenta inicialmente diferencias significativas entre cepas resistentes y susceptibles, sin embargo, con el tiempo se puede ver que en los animales resistentes se inducen fenómenos de reparación del daño, en cambio en los ratones susceptibles las lesiones parecen aumentar a pesar de no observarse parásitos intracelulares o circulantes

• English

  Males and females from A.Sn, HTG, HTI and A.Sw strains of mouse were intraperitoneally infected with 2000 trypomastigotes from Tulahuen strain of Trypanosma cruzi. All mice from A.Sn and HTG strains were equally susceptible (100% mortality) to the parasite infection, while males and females from A.Sw and HTI mouse strains were resistent (100% survival). However males from A.Sw, HTG and HTI strains showed significantly higher level of parasitemia than the corresponding females. The possible effect of parasitemia on tissue pathology and mice mortality was evaluated by comparing the histopathology of miocardial and skeleton muscle in the susceptible and resistant strains of mice. In the first ten days post infection no significant differences in tissue damage were observed between susceptible and resistent strain of mice. However in the third week of infection while A.Sw and HTI mice showed recovery of tissue lessions, the susceptible A.Sn and HTG showed increased tissue damage although no blood forms and no intracellular parasites were detected