IP3 receptors and Ca2+ signals in adult skeletal muscle satellite cells in situ

• JORDI MOLGÓ ^[1] ; CESARE COLASANTE ^[1] ; DANY S ADAMS ^[2] ; ENRIQUE JAIMOVICH ^[3]
  1. [1] Centre National de la Recherche Scientifique

     Centre National de la Recherche Scientifique

     París, Francia

     
  2. [2] Smith College

     Smith College

     City of Northampton, Estados Unidos

     
  3. [3] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 37, Nº. 4, 2004, págs. 635-639
• Idioma: inglés
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• Resumen

  • In this short article we review muscle satellite cell characteristics and our studies in adult rodent muscle satellite cells in situ. Using confocal laser scanning microscopy and immunocytochemistry, a high level of IP3 receptor (IP3R) immunostaining was detected in satellite cells. These cells were identified by their peripheral position, their size, the shape of their nucleus, the paucity of the apparent cytoplasm, and the immunostaining with specific molecular markers such as a-actinin, the neural cell adhesion molecule (N-CAM) and desmin. High extracellular K+ (60 mM) induced long-lasting Ca2+ signalsin satellite cells in situ. We suggest that electrical activity stimulates IP3-associated Ca2+ signals that could act in concert with signaling pathways triggered by growth factors and/or hormones