Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells

Victoria Velarde; Paula M de la Cerda; Claudia Duarte; Francisca Arancibia; Eduardo Abbott; Alejandra González Canacer; Francesca Moreno; Ayad A Jaffa

Ayuda

Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells

VICTORIA VELARDE ^[1] ; PAULA M DE LA CERDA ^[1] ; CLAUDIA DUARTE ^[1] ; FRANCISCA ARANCIBIA ^[1] ; EDUARDO ABBOTT ^[1] ; ALEJANDRO GONZÁLEZ ^[1] ; FRANCESCA MORENO ^[1] ; AYAD A JAFFA ^[2]
1. [1] Pontificia Universidad Católica de Chile
  
  Pontificia Universidad Católica de Chile
  
  Santiago, Chile
2. [2] Medical University of South Carolina
  
  Medical University of South Carolina
  
  Estados Unidos
Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 37, Nº. 3, 2004, págs. 419-430
Idioma: inglés
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Resumen
- In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extracellular matrix production via redox-sensitive signaling pathways. We investigated the role(s) of ROS in proliferation, migration and collagen synthesis induced by BK in VSMC derived from Sprague Dawley rat aorta. BK (10 nM) increased VSMC proliferation by 30 % (n=5); this proliferation was inhibited by the antioxidants N-acetylcysteine (20 mM) and a-lipoic acid (LA, 250 mM). In addition, BK induced an increase in cell migration and in collagen levels that were blocked by LA. ROS production induced by BK (n=10) was significantly inhibited by bisindolylmaleimide (4mM) and by PD98059 (40mM). These results suggest that: 1) ROS participate in the mechanism(s) used by bradykinin to induce cellular proliferation; 2) bradykinin induces ROS generation through a pathway that involves the kinases PKC and MEK; and 3) ROS participate in the pathways mediating cell migration and the production of collagen as a response to treatment with bradykinin. To our knowledge, this is the first report describing mechanisms to explain the participation of ROS in the cellular proliferation and extracellular matrix pathway regulated by BK