Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols

• Yuji Naito ^[1] ; Makoto Shimozawa ^[1] ; Hiroki Manabe ^[1] ; Masaaki Kuroda ^[1] ; Naoya Tomatsuri ^[1] ; Kazuhiko Uchiyama ^[1] ; Tomohisa Takagi ^[1] ; Norimasa Yoshida ^[1] ; Toshikazu Yoshikawa ^[1]
  1. [1] Kyoto Prefectural University of Medicine

     Kyoto Prefectural University of Medicine

     Kamigyō-ku, Japón

     
• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 37, Nº. 2, 2004, págs. 231-238
• Idioma: inglés
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• Resumen

  • Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol. Incubation of HAEC with oxysterols (10 muM) increased expression of adhesion molecules in a time-dependent manner. Pretreatment of HAEC with RWE at final concentrations of 1, 10, and 100 ng/ml significantly inhibited the increase of surface protein expression and mRNA levels. Adherence of monocytes to oxysterol-stimulated HAEC was increased compared to that of unstimulated cells. Treatment of HAEC with RWE significantly inhibited adherence of monocytes. These results suggest that RWE works as an anti-atherogenic agent through the inhibition of endothelial-dependent adhesive interactions with monocytes induced by oxysterols