Effects of weight loss on liver and erythrocyte polyunsaturated fatty acid pattern and oxidative stress status in obese patients with non-alcoholic fatty liver disease

Alejandra Elizondo; Julia Araya; Ramón Rodrigo; Cinzia Signorini; Cristiana Sgherri; Mario Comporti; Jaime Poniachik; Luis A Videla

Ayuda

Effects of weight loss on liver and erythrocyte polyunsaturated fatty acid pattern and oxidative stress status in obese patients with non-alcoholic fatty liver disease

ALEJANDRA ELIZONDO ^[1] ; JULIA ARAYA ^[1] ; RAMÓN RODRIGO ^[1] ; CINZIA SIGNORINI ^[2] ; CRISTIANA SGHERRI ^[2] ; MARIO COMPORTI ^[2] ; JAIME PONIACHIK ^[1] ; LUIS A VIDELA ^[1]
1. [1] Universidad de Chile
  
  Universidad de Chile
  
  Santiago, Chile
2. [2] Università degli Studi di Siena
  
  Università degli Studi di Siena
  
  Siena, Italia
Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 41, Nº. 1, 2008, págs. 59-68
Idioma: inglés
Enlaces
- Texto completo
Resumen
- Our aim was to study the influence of weight loss on the fatty acid (FA) composition of liver and erythrocyte phospholipids and oxidative stress status in obese, non-alcoholic, fatty liver disease (NAFLD) patients. Seven obese NAFLD patients who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in roux and Y were studied immediately and 3 months after surgery. Seven non-obese patients who underwent anti-reflux surgery constituted the control group. Serum F2-isoprostane levels were measured by GS/NICI-MS/MS and FA composition was determined by GC. At the time of surgery, controls and obese patients exhibited a hepatic polyunsaturated fatty acid (PUFA) pattern that correlated with that of erythrocytes. Three months after surgery, NAFLD patients lost 21% of initial body weight; serum F2-isoprostane levels decreased by 76%; total PUFA, long-chain PUFA (LCPUFA), n-3 PUFA, and n-3 LCPUFA increased by 22, 29, 81, and 93%, respectively; n-6/n-3 LCPUFA ratio decreased by 51%; docosahexaenoic acid/docosapentaenoic acid ratio increased by 19-fold; and the n-3 product/precursor ratio (20: 5 + 22: 5 + 22: 6)/18: 3 increased by 164% (p<0.05). It is concluded that weight loss improves the n-3 LCPUFA status of obese patients in association with significant amelioration in the biomarkers of oxidative stress, membrane FA insaturation, and n-3 LCPUFA biosynthesis capacity, thus representing a central therapeutic issue in the improvement of obesity-related metabolic alterations involved in the mechanism of hepatic steatosis