Effect of alternating the magnetic feld on phosphate metabolism in the nervous system of Helix pomatia

• Ljiljana M Nikolic ^[1] ; Milos B Rokic ^[2] ; Natasa V Todorovic ^[1] ; Gordana S Kartelija ^[1] ; Miodrag S Nedeljkovic ^[1] ; Joanna S Zakrzewska ^[3]
  1. [1] University of Belgrade

     University of Belgrade

     Serbia

     
  2. [2] Institute for Multidisciplinary Studies
  3. [3] Institute of General and Physical Chemistry

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 43, Nº. 2, 2010, págs. 243-250
• Idioma: inglés
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• Resumen

  • The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by 31P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na+/K+-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.