Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens

• Viviane K Graça-de Souza ^[1] ; Viviane Monteiro-Góes ^[3] ; Patrício Manque ^[2] ; Tatiana A.C.B Souza ^[1] ; Paulo R.C. Corrêa ^[1] ; Gregory A Buck ^[2] ; Andréa R Ávila ^[3] ; Lucy M Yamauchi ^[1] ; Phileno Pinge-Filho ^[1] ; Samuel Goldenberg ^[3] ; Marco A Krieger ^[3] ; Sueli F Yamada-Ogatta ^[1]
  1. [1] Universidade Estadual de Londrina

     Universidade Estadual de Londrina

     Brasil

     
  2. [2] Virginia Commonwealth University

     Virginia Commonwealth University

     Estados Unidos

     
  3. [3] Instituto Carlos Chagas FIOCRUZ

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 43, Nº. 2, 2010, págs. 233-241
• Idioma: inglés
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• Resumen

  • The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.