Integrated analyses of copy number variations and gene differential expression in lung squamous-cell carcinoma

• Zhao Yang ^[1] ; Bing Zhuan ^[2] ; Ying Yan ^[2] ; Simin Jiang ^[1] ; Tao Wang ^[1]
  1. [1] Huazhong University of Science and Technology

     Huazhong University of Science and Technology

     China

     
  2. [2] Ningxia People's Hospital Department of Respiratory and Critical Care Medicine
• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Nº. 48, 2015
• Idioma: inglés
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• Resumen

  • BACKGROUND: Although numerous efforts have been made, the pathogenesis underlying lung squamous-cell carcinoma (SCC) remains unclear. This study aimed to identify the CNV-driven genes by an integrated analysis of both the gene differential expression and copy number variation (CNV). RESULTS: A higher burden of the CNVs was found in 10-50 kb length. The 16 CNV-driven genes mainly located in chr 1 and chr 3 were enriched in immune response [e.g. complement factor H (CFH) and Fc fragment of IgG, low affinity Ilia, receptor (FCGR3A)], starch and sucrose metabolism [e.g. amylase alpha 2A (AMY2A)]. Furthermore, 38 TFs were screened for the 9 CNV-driven genes and then the regulatory network was constructed, in which the GATA-binding factor 1, 2, and 3 (GATA 1, GATA2, GATA3) jointly regulated the expression of TP63. CONCLUSIONS: The above CNV-driven genes might be potential contributors to the development of lung SCC.