Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze

• Ayyavu Mahesh ^[1] ; Devarajan Thangadurai ^[2] ; Gabriel Melchias ^[3]
  1. [1] The Volcani Center Institute of Plant Sciences Department of Plant Genetics
  2. [2] Karnatak University Department of Botany
  3. [3] St. Joseph's College Department of Plant Biology and Plant Biotechnology

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 45, Nº. 2, 2012, págs. 131-136
• Idioma: inglés
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  • An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.