Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

• Autores: Rolando García-Gonzáles, José Pico-Mendoza, Karla Quiroz, Basilio Carrasco, Pablo Cáceres, Borys Chong Pérez, Hugo Pino, Marjorie Seiltgens, Eglis Greck, Peter D. S. Caligari
• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Nº. 51, 2018
• Idioma: inglés
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• Resumen

  • Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.