Eukaryotic expression, Co-IP and MS identify BMPR-1B protein–protein interaction network
- Autores: Jianlei Jia, Jipeng Jin, Qian Chen, Zan Yuan, Haiqin Li, Junhao Bian, Linsheng Gui
- Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Nº. 53, 2020
- Idioma: inglés
- Enlaces
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Resumen
BMPR-1B is part of the transforming growth factor β super family and plays a pivotal role in ewe litter size. Functional loss of exon-8 mutations in the BMPR-1B gene (namely the FecB gene) can increase both the ewe ovulation rate and litter size. Results: This study constructed a eukaryotic expression system, prepared a monoclonal antibody, and characterized BMPR-1B/FecB protein–protein interactions (PPIs). Using Co-immunoprecipitation coupled to mass spectrometry (Co-IP/MS), 23 proteins were identified that specifically interact with FecB in ovary extracts of ewes. Bioinformatics analysis of selected PPIs demonstrated that FecB associated with several other BMPs, primarily via signal transduction in the ovary. FecB and its associated interaction proteins enriched the reproduction process via BMP2 and BMP4 pathways. Signal transduction was identified via Smads proteins and TGF-beta signaling pathway by analyzing the biological processes and pathways. Moreover, other target proteins (GDF5, GDF9, RhoD, and HSP 10) that interact with FecB and that are related to ovulation and litter size in ewes were identified. Conclusions: In summary, this research identified a novel pathway and insight to explore the PPi network of BMPR-1B.
