A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads

• Gabriel León Fiszman ^[1] ; Armando Luis Karara ^[1] ; Liliana María Elena Finocchiaro ^[1] ; Gerardo Claudio Glikin ^[1]
  1. [1] UBA Instituto de Oncología A.H. Roffo Unidad de Transferencia Genética
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 5, Nº. 3, 2002, págs. 23-24
• Idioma: inglés
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• Resumen

  • The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.