Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish (Danio rerio)

Reynold Morales ^[3] ; María Teresa Herrera ^[1] ; Amílcar Arenal ^[2] ; Asterio Cruz ^[4] ; Oscar Hernández ^[2] ; Rafael Pimentel ^[2] ; Isabel Guillén ^[2] ; Rebeca Martínez ^[3] ; Mario P Estrada ^[3]
1. [1] Universidad de La Habana
  
  Universidad de La Habana
  
  Cuba
2. [2] Centro de Ingeniería Genética y Biotecnología
  
  Centro de Ingeniería Genética y Biotecnología
  
  Cuba
3. [3] Center for Genetic Engineering and Biotechnology Mammalian Cell Genetics Division
4. [4] Center for Genetic Engineering and Biotechnology Division of Quality Control and Assurance
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Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 4, Nº. 2, 2001, págs. 5-6
Idioma: inglés
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Resumen
- Gene transfer is economically important and model fish species has produced a great impact in modern biology and biotechnology. Transgenic zebrafish (Danio rerio) were generated through the co-injection of a GFP-expressing plasmid and an "all fish" transgene composed by the carp beta-actin promoter and the chromosomal tilapia (Oreochromis hornorum) growth hormone gene. The GFP expression was a good indicator of stable transformation and allowed for high efficiency selection of transgenic fish. Transgenic F1 zebrafish grew 20% faster than full sibling non-transgenic controls.