Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility

• Kamolnan Lakrod ^[1] ; Chulee Chaisrisook ^[1] ; Daniel Z Skinner ^[2]
  1. [1] Kasetsart University

     Kasetsart University

     Tailandia

     
  2. [2] Washington State University

     Washington State University

     Estados Unidos

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 6, Nº. 2, 2003, págs. 143-147
• Idioma: inglés
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• Resumen

  • Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus.