A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database

• B Jayashree ^[1] ; Hutokshi K Buhariwalla ^[2] ; Sanjeev Shinde ^[1] ; Jonathan H Crouch ^[2]
  1. [1] International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Bioinformatics and Computational Biology Unit
  2. [2] International Crops Research Institute for the Semi- Arid Tropics (ICRISAT) MS Swaminathan Applied Genomics Lab
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 8, Nº. 2, 2005, págs. 8-13
• Idioma: inglés
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• Resumen

  • Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different sources of drought avoidance and tolerance (ICC4958 and Annigeri respectively). This database provides researchers in legume genomics with a major new resource for data mining associated with root traits and drought tolerance. This report describes the development and utilization of the database and provides the tools we have developed to facilitate the bioinformatics pipeline used for analysis of the ESTs in this database. We also discuss applications that have already been achieved using this resource