Modification of the activity of an alpha-amylase from Bacillus licheniformis by several surfactants

• Vicente Bravo Rodríguez ^[1] ; Encarnación Jurado Alameda ^[1] ; Juan Francisco Martínez Gallegos ^[1] ; Antonia Reyes Requena ^[1] ; Ana Isabel García López ^[1] ; Joaquim Manuel Sampaio Cabral ^[2] ; Pedro Fernandes ^[2] ; Luis Joaquim Pina da Fonseca ^[2]
  1. [1] Universidad de Granada

     Universidad de Granada

     Granada, España

     
  2. [2] Universidade de Lisboa

     Universidade de Lisboa

     Socorro, Portugal

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 9, Nº. 5, 2006
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant significantly depressed enzymatic activity, presumably due to the electrostatic interactions caused by its anionic character.