Resumen de Isolation of simple sequence repeats from groundnut

Chuan Tang Wang, Xin Dao Yang, Dian Xu Chen, Shan Lin Yu, Guang Zhen Liu, Yue Tang, Jian Zhi Xu

• SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts