Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions

• Susana Rasmussen-Poblete ^[1] ; Jorge Valdes ^[1] ; Maria Cecilia Gamboa ^[1] ; Pablo D.T Valenzuela ^[1] ; Erwin Krauskopf ^[1]
  1. [1] Universidad Andrés Bello

     Universidad Andrés Bello

     Santiago, Chile

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 2, 2008, págs. 56-68
• Idioma: inglés
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• Resumen

  • Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.