Genetic and physical map of broad host range cosmid pRG930cm

• Andrew Chibeu ^[1] ; Rob Lavigne ^[2] ; Wesley Mattheus ^[2] ; Sandra Matthijs ^[1] ; Pierre Cornelis ^[1] ; Guido Volckaert ^[2]
  1. [1] Vrije Universiteit Brussel

     Vrije Universiteit Brussel

     Arrondissement Brussel-Hoofdstad, Bélgica

     
  2. [2] KU Leuven

     KU Leuven

     Arrondissement Leuven, Bélgica

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 12, Nº. 2, 2009, págs. 10-11
• Idioma: inglés
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• Resumen

  • We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.